Extended Data Fig. 10: Absence of SYNV vector in M1 progeny derived from various regenerated M0 lines.
From: Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
Total RNA extracted from four or five individual M1 plants derived from representative M0 lines were analyzed by RT-PCR with SYNV- and actin-specific primer sets. Inf. indicates an RNA sample extracted from an SYNV-infected plant serving as a positive control. M, 1-kb ladder DNA marker.
