Extended Data Fig. 1: Stability of SYNV vectors after mechanical transmission.
From: Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
a, GFP fluorescence images of N. benthamiana 16c plants mechanically inoculated with V-Cas9 or V-gGFP1-Cas9. Photographs were taken under long-wavelength UV illumination at 30 days after systemic infection. b, PCR-RE detection of mutation frequencies in plants mechanically infected by V-gGFP1-Cas9. V-Cas9/U and V-Cas9/D denote a V-Cas9-infected plant without or with restriction digestion. M, 1-kb ladder DNA marker. c, RT-PCR detection of gRNA and Cas9 inserts in upper leaves of plants systemically infected by SYNV derivatives. V-WT, wild-type SYNV infection; d, Western blot analysis of the expression of the viral structural proteins, Cas9 and GFP in infected leaf tissues. The Coomassie Brilliant Blue-stained large subunit of RuBisCO (rbcL) severs as protein loading control.
