Extended Data Fig. 3: Mutagenesis frequencies induced by tRNA-processed and unprocessed gRNAs.
From: Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
a, PCR-RE detection of mutagenesis frequency. DNA fragments containing the gGFP2 target site were amplified by PCR from chromosomal DNA extracted from plants infected with SYNV-gRNA-Cas9 or SYNV-tgtRNA-Cas9 and digested by NcoI. Mock/U and Mock/D denote a mock-infected plant without or with restriction digestion. b, Sanger sequencing results of target site mutations induced by SYNV-gRNA-Cas9. Base substitution is italicized, and base deletions are denoted by dashes, with the number of base substitution (s#) and base deletion (d#) indicated on the right of each sequence. Examples of sequencing chromatographs are shown.
