Extended Data Fig. 2: Phenotypic and molecular characterization of the xiaomi-2 mutant.
From: A mini foxtail millet with an Arabidopsis-like life cycle as a C4 model system
a, Forty-day-old plants of Jingu21 (wild type, left) and xiaomi-2 (right) plants grown under natural long-day conditions. b, Heading date of Jingu21 and xiaomi-2 under natural field conditions. The heading date of ≥ 20 plants was measured for each replicate (n = 3 biologically independent replicates, ≥ 102 in total). The bottom and top of boxes represent the first and third quartile, respectively. The middle line is the median and the whiskers represent the maximum and minimum values. Statistical analysis was performed using two-tailed Wilcoxon rank-sum test. c, A mature small-sized xiaomi-2 plant (right) compared to Jingu21 (left), at the 68th day in field. d, Plant height of Jingu21 and xiaomi-2 under natural field conditions. The plant height of ≥ 23 plants was measured for each replicate (n = 3 biologically independent replicates, ≥ 83 in total). e, Molecular charicterization of xiaomi-2. Exons and introns are denoted by filled boxes and lines, respectively. P2F and P2R represent a pair of primers used to amplify the fragments harboring the mutation site from the segregating M3 individuals (Primer sequences are listed in Supplementary Table 3). f, Structure of PHYC and its mutation version deduced according to mutations in xiaomi-2. Scale bars, 10 cm in a and c.
