Extended Data Fig. 4: Gene expression in transgenic wheat plants overexpressing single splice variants of the Pm4b gene. | Nature Plants

Extended Data Fig. 4: Gene expression in transgenic wheat plants overexpressing single splice variants of the Pm4b gene.

From: Wheat Pm4 resistance to powdery mildew is controlled by alternative splice variants encoding chimeric proteins

Extended Data Fig. 4

a, Expression levels of Pm4bV1_CDS transgenes in selected T1 progeny for three independent transgenic events (T1#9, T1#12, T1#12) overexpressing full-length cDNA of Pm4b_V1 compared to the endogenous Pm4b_V1 transcripts in the wild-type Fed-Pm4b (second bar). b, Expression levels of Pm4bV2_CDS transgenes in selected T1 progeny for three independent transgenic events (T1#6, T1#24, T1#29) overexpressing full-length cDNA of Pm4b_V2 compared to the endogenous Pm4b_V2 transcripts in the wild-type Fed-Pm4b (second bar). For a and b, data points are technical replicates (triple quantifications) on single T1 progeny. Error bars, mean ± s.e.m. of three technical replicates. On top of each bar, the number corresponds to the x-fold expression compared to Pm4b_V1 or Pm4b_V2 in the wild-type Fed-Pm4 genotype. Below each T1 progeny, representative images of disease reactions after infection with the Pm4a/b-avirulent Bgt96224 and Bgt94202 isolates are shown.

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