Extended Data Fig. 9: Binding ability of Pm4b variants for homo- and heteromeric interactions. | Nature Plants

Extended Data Fig. 9: Binding ability of Pm4b variants for homo- and heteromeric interactions.

From: Wheat Pm4 resistance to powdery mildew is controlled by alternative splice variants encoding chimeric proteins

Extended Data Fig. 9

a, Split-LUC combinations showing luciferase signal for Pm4b_V1 homomeric interaction in Fig. 4e were co-infiltrated with fluorescence-tagged Pm4b_V2 protein variants. b, Split-LUC combinations showing luciferase signal for Pm4b_V2 homomeric interaction in Fig. 4f were co-infiltrated with fluorescence-tagged Pm4b_V1 protein variants. The data are displayed following the same logic as presented in Fig. 4: in each of the 18 panels, the first boxplot corresponds to the positive control, AvrPm3b_N-LUC & AvrPm3b_C_LUC. The second boxplot (orange colour) corresponds to the tested combination, displayed at the top of each panel. For simplicity, V1 and V2 refer to Pm4b_V1 and Pm4b_V2, respectively. Finally, the last two boxplots in each panel correspond to the negative controls co-infiltrated. Significant differences were determined by Krustal–Wallis test followed by Dunn’s multiple comparisons test with two-sided 95.0% confidence interval with Bonferroni correction based on n = 24 (8 technical and 3 biological replicates). Exact P values are shown above bars. In the boxplots, centre lines show the medians; box limits indicate the 25th and 75th percentiles as determined by the geom_boxplot function of the ggplot2 R package; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, individual data points are represented by dots.

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