Extended Data Fig. 8: Effects on endocytosis of the depletion of PI(4,5)P2 using the iDePP system.
From: Inducible depletion of PI(4,5)P2 by the synthetic iDePP system in Arabidopsis
a,b, Representative confocal images of Arabidopsis root cells coexpressing mCIT-P4MSidM with either MAP-mCH-dOCRL (a) or MAP-3xmCH (b). c,d, Confocal imaging showing the effect of the expression of MP-mCH-dOCRL on CLC2-GFP localization. e, Quantification of the effect of MAP-mCH-dOCRL expression on CLC2-GFP localization. The dissociation index is the ratio of (i) plasma membrane to cytosol fluorescence ratio without dex treatment, (ii) plasma membrane to cytosol fluorescence ratio after dex treatment. Statistical analysis with LMER (Type II Wald χ2 test) and posthoc tests; 3 replicates (f-g) Confocal imaging showing the effect of the expression of MAP-mCH-dOCRL on SH3P2-sGFP line (h) Quantification of the effect of MAP-mCH-dOCRL expression on SH3P2-sGFP localization. Per root, cells showing a delocalization of SH3P2-sGFP were manually counted for untreated and dex treated plants using FIJI ‘multi-point’ tool and compared by a generalized linear model, Poisson family; 3 replicates. In the plots, middle horizontal bars represent the median, while the bottom and top of each box represent the 25th and 75th percentiles, respectively. At most, the whiskers extend to 1.5 times the interquartile range, excluding data beyond. For range of value under 1,5 IQR, whiskers represent the range of maximum and minimum values. All statistical tests were two-sided. Scale bar, 10 μm.
