Extended Data Fig. 4: Biochemical quantification of the PIP2, PIP and PA after iDePP induction.

The ³²P-PIP levels in iDePP lines and controls ± dex of 3 independent replicates. a-c, Seedlings were labelled with ³²_Pi and incubated ± dex O/N (16–20 hrs). Each sample contained the lipid extract of three seedlings, of which 1/5^th was analyzed by TLC and quantified by phosphoimaging, of which ³²P-PIP₂ (a), PIP (b) and PA (c) were calculated as percentage of total ³²P-lipids. d-f, Time-course analysis of ³²P-PIP₂ (d), PIP (e) and PA (f) levels in iDePP seedlings ± dex in MAP-mCH-dOCRL line. Seedlings of MAP-mCH-dOCRL line were labelled for 20 hrs with ³²_Pi and co-incubated with or without dex for the times indicated (0–20 hrs). Each sample contained the lipid extract of three seedlings of which 1/5^th was analyzed by TLC and quantified by phosphoimaging. First, the mean of control on all time points was calculated. The graphs represent the calculated mean of the sample reported to the mean of the control. In the plots, middle horizontal bars represent the median, while the bottom and top of each box represent the 25th and 75th percentiles, respectively. At most, the whiskers extend to 1.5 times the interquartile range, excluding data beyond. For range of value under 1,5 IQR, whiskers represent the range of maximum and minimum values. All statistical tests were two-sided.