Extended Data Fig. 3: Commensals can degrade/modify flg22 in vitro.
From: Coordination of microbe–host homeostasis by crosstalk with plant innate immunity
a, Schematic diagram showing how bacterial supernatants are collected. Root lengths of plants coinoculated with the supernatant of different bacteria and b, 1 μM flg22 (pWER::FLS2-GFP) or c, 1 μM Atpep1 (Col-0). All strains used in these experiments exhibited RGI suppressive activity as shown in Fig. 1. d, Root lengths of Col-0 plants treated with the supernatant of Janibacter 101 after different treatments shown in (a). e, Mass spectrometry analyses detecting the intact flg22 peptide (QRLSTGSRINSAKDDAAGLQIA [M + 4H] 568.8056) by estimating the peak area generated by Skyline. All strains used in this experiment exhibited suppressive activity on flg22-mediated RGI. n=total number of biological samples collected from three biological replicates (ED Fig. 3b–e). Asterisks or different letters indicate statistical significance (two-sided Dunn’s Kruskal Wallis or two-sided Dunnett’s test, p < 0.05). The box plots centre on the median and extend to the 25th and 75th percentiles, and the whiskers extend to the furthest point within the range of 1.5× the interquartile range.
