Fig. 1: Golden Gate assembly system of plant organelle base editors. | Nature Plants

Fig. 1: Golden Gate assembly system of plant organelle base editors.

From: Chloroplast and mitochondrial DNA editing in plants

Fig. 1

Schematic illustration of Golden Gate assembly for cp-DdCBE and mt-DdCBE construction. For each position in a target sequence, TALE subarray plasmids were selected from a total set of 424 (6 × 64 tripartite + 2 × 16 bipartite + 2 × 4 monopartite) and mixed with a destination vector to generate plasmids encoding DdCBEs targeted to specific sequences.

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