Extended Data Fig. 5: Subcellular localization of Trx-h2 in Arabidopsis protoplasts at warm temperature.
From: Redox-dependent structural switch and CBF activation confer freezing tolerance in plants
a, A schematic diagram of the construct used to express Trx-h2-YFP under the control of the CaMV 35S promoter in Arabidopsis protoplasts. b, Subcellular location of Trx-h2 in Arabidopsis protoplasts at warm temperature (22 °C). YFP signal was detected by confocal microscopy. Other fluorescent makers including mCherry-HDEL, soybean α-1,2-mannosidase 1-RFP, and NLS-RFP were used to label the endoplasmic reticulum (ER), Golgi complex, and nucleus, respectively, in the second lane (RFP). The panel labeled ‘Merge’ represents overlapped images of YFP and RFP signals. Bright field images are presented in the panel labeled ‘Bright’. Scale bars = 20 μm.
