Extended Data Fig. 6: HvMADS1 represses cell division in the spike in response to high temperature.
From: MADS1 maintains barley spike morphology at high ambient temperatures
a, Indicative method of EdU (5-ethynyl-2’-deoxyuridine) tracking in barley spike. PI, propidium iodide. b,c, EdU tracking of cell division activities in WT (GP) and hvm1 spikes (W2.5 and W3–3.5) grown at 15 °C (b) and 25 °C (c). White arrows indicate ectopic EdU clusters in non-floret meristem regions of the central spikelet, which shows high levels of cell division activity in ectopic meristems of hvm1 plants. Numbers of ectopic clusters represent the average observed additional EdU signal clusters in non-floret meristem regions per spike (W3–3.5). Also see the Source Data. Bars = 100 µm. The pictures of EdU tracking assays represent one of three experiments performed independently with similar results.
