Extended Data Fig. 7: Temperature does not alter HvMADS1 mRNA expression or protein accumulation.
From: MADS1 maintains barley spike morphology at high ambient temperatures
a, Accumulation of the HvMADS1 protein in flower organs, including lemma, palea, anther, and lodicule, in pro::HvMADS1-eGFP transgenic lines at 15 °C. BF, bright field. Bars = 100 µm. b, qRT-PCR analysis of HvMADS1 expression in W2.5 and W3.5 WT spikes at different temperatures. A temperature-responsive gene (HB, homeobox) served as the positive control. Data shown as mean ± s.d., n = 3 biological replicates. c, RT-PCR analysis of HvMADS1 expression in WT spikes (W3.5). HvActin7 served as the control. kb indicates kilobase. d, Immunoblot analysis of HvMADS1-eGFP protein in W3.5 spikes from three independent pro::HvMADS1-eGFP transgenic lines in response to temperatures. Tubulin served as a loading control. All experiments were repeated independently at least three times with similar results.
