Extended Data Fig. 1: Creation of barley sep mutants using CRISPR–Cas9.
From: MADS1 maintains barley spike morphology at high ambient temperatures
a, The gene structure of HvMADS1 and positions of two sgRNA targets (T1 and T2) for CRISPR–Cas9 editing in the MADS-box domain. Blue rectangles indicate exons of HvMADS1. b, DNA sequences of independent T0 transgenics of Hvmads1 (hvm1) mutants in GP, WI, and Vla backgrounds, and hvm1/5 and hvm1/34 double mutants in GP, carrying putative HvMADS1 biallelic and homozygous mutations. WT, wild-type. c, The putative amino acid sequences encoding HvMADS1 of hvm1 single mutant, and hvm1/5 and hvm1/34 double mutants [from (b)]. Asterisks indicate a stop codon. d,e, Genotypes of three independent lines of two sgRNA targets of HvMADS5 (d) and HvMADS34 (e) in hvm5 and hvm34 single mutants, and hvm1/5, hvm1/34 and hvm5/34 double mutants that were used for CRISPR–Cas9 editing, respectively.
