Extended Data Fig. 8: Identification of a Type I isoform replacing LhcbM1 in the structure of PSI-LHCI-LHCII supercomplex from the ΔLhcbM1 mutant strain.
From: Structural basis of LhcbM5-mediated state transitions in green algae
Map features of characteristic residues and the corresponding sequence (residues are numbered according to LhcbM1). The density of the LHCII-1 region of PSI-LHCI-LHCII map is shown, with local resolution of approximately 3.75 Å and contour level of 1.31 rmsd. Type I LhcbM proteins have a Trp48 (residue 49 in LhcbM1) in the N-terminal regions, whereas Type III and Type IV have a Phe in the corresponding position, therefore can be excluded. Residue Tyr52 (residue 53 in LhcbM1) shows clear density for its side-chain, which excludes the possibility of Type II LhcbM protein as LhcbM5 has a Leu at the corresponding position. The map features of Tyr181 further verify the assignment of Type I isoform. Unique residues used for identification are indicated by red arrows.
