Extended Data Fig. 4: Expression pattern of PtrPHBMT1 in Populus.

a, Relative expression levels of PtrPHBMT1 was determined via RT–qPCR using RNAs from P. trichocarpa leaf, root, stem (> 1 year), shoot apex, and the bark from the young stem (<3 months), the developing xylem scrapped from young stem (<3 months), the debarked young (<3 months) and old wood (>1 year). Data are presented as means ± S. D. of three biological repeats. The relative expression levels were referenced with ubiquitin gene. The expression level in the old wood sample was set as 100%. b, In silico gene expression pattern of PtrPHBMT1. The data were retrieved from PlaNet (http://aranet.sbs.ntu.edu.sg/) using Probset ID PtpAffx.59062.1.A1_at. Data are presented as means ± S. D. of signal values from three individual microarray experiments.