Extended Data Fig. 5: Subcellular localization of PtrPHBMT1.
From: Monolignol acyltransferase for lignin p-hydroxybenzoylation in Populus
a, b, Fluorescence distribution of the free GFP(a) and PtrPHBMT1–GFP fusion protein (b) transiently expressed in N. benthamiana leaf cells. c, d, Plasmolysis analysis for GFP (c) and PtrPHBMT1–GFP (d) transiently expressed in N. benthamiana leaf cells. The GFP fluorescence in green and chlorophyll fluorescence in red. Bar = 20 μm. Arrows point out the cell wall region. e, Immunoblots of PHBMT1 in the soluble (S), microsomal (MS), and cell (CW) protein fractions using anti-PHBMT1 antibody. 20 µg proteins were loaded in each lane. M, marker. The experiments in (a–e) were repeated twice independently with similar results.
