Extended Data Fig. 6: DNA sequences of PHBMT1 target site and the potential off-target site of its close homologous gene in the obtained CRISPR/Cas9 transgenic lines.
From: Monolignol acyltransferase for lignin p-hydroxybenzoylation in Populus
The homozygous, biallelic, and chimeric mutations were determined according to the allelic sequences of the target site compared to Populus PHBMT1 reference sequence. PAM sequence(AGG) is highlighted. The edited events usually happen after -3 position of PAM (g1-8, g1-9, g1-11). The mutations are occurred in the targeted PtxaPHBMT1 gene but not in its homologue Ptxa001G447300.
