Extended Data Fig. 8: Analysis of PHBMT1 overexpression hybrid aspens.

a, Growth phenotype of 6-month-old PtrPHBMT1 overexpression aspens (OEs). Bar = 30 cm. b, Immunoblots probed with anti-PHBMT1 antibody on the crude proteins from the WT and PHBMT1 OE transgenic aspens. The immunoblots against anti-actin antibody served as the loading control. The original blots are presented in Supplementary Fig. 5b and d. The experiments were repeated twice independently with similar results. c, d, Stem height (c) and basal stem diameter (d) of the 3- and 5-month-old WT and OE lines. The data in (c, d) are presented as means ± S. D. of four (WT and OE3) and seven (OE1) biological repeats. e, Total acetyl bromide soluble lignin content of 6-month-old stems. The data are presented as means ± S. D. of three biological repeats. f, Woody biomass yield of the 6-month-old hybrid aspens. g, The calculated wood density of the basal stems of 6-month-old plants. The data in (f, g) are presented as means ± S. D. of three (WT and OE3) and four (OE1) biological repeats. No statistical differences were found relative to the WT.