Extended Data Fig. 9: Detection of sinapyl p-hydroxybenzoate (SA-pBA) from poplar lignin fraction via DFRC.
From: Monolignol acyltransferase for lignin p-hydroxybenzoylation in Populus
a–c, Extract ion chromatography of the DFRC products from the prepared cellulolytic enzyme lignin of the PHBMT1 overexpression line OE1(a), the WT (b) and the PHBMT1 knockout mutant g1-8 (c), The ions were scanned at m/z = 372 for 4-O-acetylsinapyl-p-O-acetylbenzoate. Note the different ion abundance scale in a–c. d, The full mass spectra of the peak at retention time 39 min in a, b which is absent in c. e, Schematic diagram of 4-O-acetylsinapyl-p-O-acetylbenzoate, the DFRC product of SA-pBA, and its characteristic ions.
