Extended Data Fig. 2: The apparent optimal temperature and pH value of buffer system of PtrPHBMT1(PtACT54)-catalysed reactions.
From: Monolignol acyltransferase for lignin p-hydroxybenzoylation in Populus
a, b, The pH preference of PtrPHBMT1(PtACT54) with p-hydroxybenzoyl-CoA (a) or acetyl-CoA (b) and sinapyl alcohol substates. c, d, The optimal temperature for PtrPHBMT1(PtACT54)-catalysed reaction with p-hydroxybenzoyl-CoA (c) or acetyl-CoA (d) and sinapyl alcohol substrates. The data in (a–d) are presented as means ± S. D. of three (b) or four (a, c, d) experimental repeats. In (a, b) the measured activity at pH 5 was set as 100%. In (c and d) the activity at 20 °C was set as 100%.
