Extended Data Fig. 8: Expression of RLP42 gene and function analysis of RLP42 alleles from selected A. thaliana accessions.
From: Distinct immune sensor systems for fungal endopolygalacturonases in closely related Brassicaceae
a, Quantitative (upper panel, qRT-PCR) and semi-quantitative (lower panel) real-time PCR analysis of RLP42 expression. Ciste-1, Gu-0, Shigu-1, and Col-0 are sensitive (+) to pg13(At), whereas Dobra-1, Ler-1, NFA-10, Petro-1, Ts-5, and Tsu-1 are insensitive (-) to pg13(At). For qRT-PCR, relative expression of RLP42 was normalized to the levels of EF-1α transcript. Genomic DNA (gDNA) from Col-0 served as positive control. Bars represent means ± standard deviation of three replicates. Data points are indicated as black dots. Assays were performed in duplicate with similar results. b, Ethylene accumulation after 4 h treatment with water (mock), 1 µM flg22, or 1 µM pg9(At) in N. benthamiana leaves transiently transformed with RLP42 (Col-0) derivatives carrying RLP42 (Petro-1)-specific point mutations or RLP42 alleles from accessions Petro-1 and Dobra-1. Data points are indicated as grey dots from three independent experiments [n = 9, except for RLP42, RLP42 (Petro-1), n = 12] and plotted as box plots (centre line: median, bounds of box: the first and third quartiles, whiskers: 1.5x the interquartile range, error bar: minima and maxima). Statistically significant differences to the response observed in RLP42-expressing plant are indicated (two-sided Student’s t-test). c, Immunoblot analysis using anti-GFP antibody of protein extracts from N. benthamiana leaves transiently expressing RLP42 mutant and allelic proteins. Assays were performed in duplicate with similar results.
