Extended Data Fig. 1: Ethylene-inducing activity of Botrytis cinerea PGs and peptides derived from PGs.
From: Distinct immune sensor systems for fungal endopolygalacturonases in closely related Brassicaceae
Ethylene accumulation in A. thaliana Col-0 was measured 4 h after treatment with the following elicitors. a, water (mock), 5 mM DTT, or 60 nM DTT untreated/treated PG3/PG6. b,c, water (mock), Glu-C (b)/Lys-C (c), or 100 nM Glu-C (b) or Lys-C (c) untreated/treated PG3/PG6. d, water (mock), 50 nM PG6, or 1 or 10 µM synthetic peptides pep1-4. e, water (mock), 10 nM pg9(At) alone or together with 100 µM mutagenized pg13N186A, pg13D188A, pg13D188K, pg13F190A, or pg13F190W. f, water (mock), 1 µM pg9(At), Oom-pg9 (AKNTDGFDL, derived from PGs of Phytophthora sp.), or Bac-pg9 (AKNTDGFDP, derived from PGs of Xanthomonas sp.). Bars represent means ± standard deviation of three replicates. Data points are indicated as black dots. Statistically significant differences to mock treatments are indicated (two-sided Student’s t-test). ns, no significant difference to pg9(At) treatment.
