Extended Data Fig. 5: Fluc activity in protoplasts from wild-type (Col-0), ago1-27, rdr6-15, and rdr2-1 plants and transformed with the pUBQ10:PHO2-UTR-Fluc.
From: Exogenous miRNAs induce post-transcriptional gene silencing in plants
The activity of Fluc was higher in the ago1-27, as expected from the lack of repression of the PHO2 target sequence by the endogenous miR399. Panel a refers to the experiment reported in Fig. 5a, while panel b refers to the experiment reported in Fig. 5b. Both experiments were conducted without the addition of exogenous RNA. In the box-blots, the dots represent the single data points, whiskers denote the min/max values, the box defines the interquartile range, centre represents the median, box bounds represent the lower and upper quartiles. Different letters (a,b,c) refer to differences in ANOVA tests (Tukey’s post‐hoc test, P < 0.05). t (a: n = 4 biological replicates; b: n = 5 biological replicates).
