Extended Data Fig. 6: The exogenous miR156 signalling pathway requires both AGO1 and RDR6.
From: Exogenous miRNAs induce post-transcriptional gene silencing in plants
a. Feeding of exogenous synthetic miR156 to wild-type protoplasts transiently transformed with 35S:SPL9-Fluc, together with a 35S:Rluc plasmid for normalization. b. Feeding of exogenous synthetic miR156 (0.2 nM) to protoplasts from wild-type (Col-0), ago1-27, rdr6-15, and rdr2-1 plants transformed with the 35S:SPL9-Fluc, plus a 35S:Rluc vector as internal control. In the box-blots, the dots represent the single data points, whiskers denote the min/max values, the box defines the interquartile range, centre represents the median, box bounds represent the lower and upper quartiles. Different letters (a,b) refer to differences in ANOVA tests (Tukey’s post‐hoc test, P < 0.05). Welch’s t test (two sided) values are shown (a: n = 6 biological replicates; b: n = 5 biological replicates).
