Extended Data Fig. 7: The exogenous miR399 signalling pathway does not require DCL1 and HYL1.
From: Exogenous miRNAs induce post-transcriptional gene silencing in plants
Feeding of exogenous synthetic miR399 to wild-type protoplasts transiently transformed with the pUBQ10:PHO2-UTR-Fluc together with a 35S:Rluc plasmid for normalization. The activity of Fluc was higher in the dcl1-9 and in hyl1, as expected from the lack of repression of the PHO2 target sequence by the endogenous miR399, which cannot be correctly processed in the absence of DCL1 and HYL1. Feeding the synthetic miR399 reduces the Fluc activity indicating successful repression of the PHO2 miR399-target sequence. In the box-blots, the dots represent the single data points, whiskers denote the min/max values, the box defines the interquartile range, centre represents the median, box bounds represent the lower and upper quartiles (n = 6 biological replicates). Welch’s t test (two sided) values are shown.
