Fig. 4: Responses to exogenous RNA in protoplasts from pUBQ10:PHO2-UTR–Fluc leaves.
From: Exogenous miRNAs induce post-transcriptional gene silencing in plants
a, Effect of exogenous RNA (0.1 μg) from WT and OxmiR399 plants (added to a total volume of 2 ml) in pUBQ10:PHO2-UTR–Fluc protoplasts treated with PEG 4000 to facilitate entry of the miRNAs (n = 5 biological replicates). b, Feeding of exogenous RNA (0.1 μg) from WT and OxmiR399 plants to pUBQ10:PHO2-UTR–Fluc protoplasts (added to a total volume of 2 ml) (n = 5 biological replicates). c, Transformation of protoplasts from OxmiR399 and Col-0 plants with pUBQ10:PHO2-UTR–Fluc or pUBQ10:random-UTR–Fluc (n = 4 biological replicates). d, Feeding of exogenous RNA (0.1 μg, added to a total volume of 2 ml) from OxmiR399 plants to WT protoplasts transformed with pUBQ10:PHO2-UTR–Fluc or pUBQ10:random-UTR–Fluc (n = 5 biological replicates). In the boxplots, dots represent single data points, whiskers denote minimum/maxmum values, the box defines the interquartile range, the centre represents the median and box bounds represent the lower and upper quartiles. Welch’s t-test (two-sided) values are shown. Different letters (a,b,c) indicate differences in ANOVA tests (Tukey’s post‐hoc test, P < 0.05).
