Fig. 7: Plants overexpressing miR399 influence the expression of PHO2 in WT plants sharing the same hydroponic medium.
From: Exogenous miRNAs induce post-transcriptional gene silencing in plants
a, Presence and quantity of miR399 in the external medium of WT and OxmiR399 plants (n = 4 biological replicates). The miRNA amount is expressed using the value resulting after subtracting the actual cycle threshold (Ct) value in PCR from the arbitrary value of 40 (40-Ct). b, Expression of PHO2 in WT plants, OxmiR399 plants, WT plants cocultivated with OxmiR399 plants in the same hydroponic tray and OxmiR399 plants cocultivated with WT plants in the same hydroponic tray (n = 5 biological replicates). c, Expression of SPL3 and SPL9 in WT plants, OxmiR399 plants, WT plants cocultivated with OxmiR399 plants in the same hydroponic tray and OxmiR399 plants cocultivated with WT plants in the same hydroponic tray (n = 5 biological replicates). d, Luciferase activity in pPHO2:Fluc plants cocultivated with OxmiR399 plants in the same hydroponic tray (n = 8 biological replicates). e, Fluc and PHO2 mRNA levels in pPHO2:Fluc plants cocultivated with OxmiR399 plants in the same hydroponic tray (n = 8 biological replicates). f, Luciferase activity in pPHO2:Fluc plants cocultivated with 35S:ami-Fluc and OxmiR399 plants in the same hydroponic tray (n = 8 biological replicates). g, Fluc and PHO2 mRNA levels in pPHO2:Fluc plants cocultivated with 35S:ami-Fluc and OxmiR399 plants in the same hydroponic tray (n = 8 biological replicates). In the boxplots, dots represent single data points, whiskers denote minimum/maximum values, the box defines the interquartile range, the centre represents the median and box bounds represent the lower and upper quartiles. Welch’s t-test (two-sided) values are shown. Different letters (a,b,c) indicate differences in ANOVA tests (Tukey’s post‐hoc test, P < 0.05).
