Extended Data Fig. 1: The agronomic traits of msca1 and its paralog mutants.
a, The knock-out mutations (red asterisks on the gene model) for ZmGRX2 and ZmGRX5 were caused by CRISPR/Cas9 gene editing. zmgrx2-1 and zmgrx2-2 contain 55 bp and 70 bp deletions before the conserved CCMC motif, respectively. zmgrx5-1 contains 13 bp deletion within the CCMC motif, and zmgrx5-2 harbors 5 bp deletion before the motif. b, Mutations in MSCA1 and its paralogs have effects on the plant architecture, with the msca1;zmgrx5-1 double and grx triple-1 (msca1;zmgrx2-1;zmgrx5-1) mutants being obviously shorter. c-f, The plant height (c), kernel row number (d), tassel branch number (e) and spikelet density (f) of msca1;zmgrx5 and grx triple were significantly decreased in comparison to wild type (WT). Data are shown as mean ± SD, dots show data distribution. (n > 10 biologically independent samples, P values calculated using two-tailed t-test). g-i, grx triple-2 (msca1;zmgrx2-2;zmgrx5-2) mutants were shorter (g), with less tassel branches (h), and had smaller ears with less kernels (i) in comparison to wild type plants. Scale bars, 30 cm in b and g, 5 cm in h and i.
