Extended Data Fig. 1: Expression of miR156/7 in Arabidopsis.
From: A robust mechanism for resetting juvenility during each generation in Arabidopsis
a, Expression of miR156/7 and pMIR156C:GFP-N7 reporter in the shoot apex and embryos. Plants were grown under LD conditions. The shoot apices of 6- or 18-day-old plants and the embryos at the bent cotyledon stage were harvested and examined. For the shoot apices samples, all the leaves larger than 0.5 mm in length were removed. The expression level in the shoot apex at 6 d was set to 1.0. The results were represented by mean ± sem, n = 4. The statistical analysis is shown in Supplementary Table 1. b, Schematic diagram of miR156/7 sensor. The GFP-N7 was driven by the promoter of RIBO (AT2G18020), and followed by the 3’ UTR of SPL3 which contains a miR156/7 target site (red line). c, Periodic expression of miR156/7* during alternation of generations. The abundance of miR156a*-d*, miR156f*, miR157a*/b* and miR157c* was used to infer the periodic expression of miR156/7 during alternation of generations. miR157a/b have the same miRNA* sequence (miR157a*/b*). The star sequence for miR156e was not available. The results were represented by mean ± sd. For juvenile leaf, adult leaf and pollen samples, n = 3; for other tissue samples, n = 2. Please note that miR156a-c are highly abundant within the miR156 family. See also Fig. 1c. The original data for miR156/7 abundance are shown in Supplementary Table 2.
