Extended Data Fig. 6: Strong PIN1 expression is associated with sites of auxin biosynthesis.
From: Specification of leaf dorsiventrality via a prepatterned binary readout of a uniform auxin input
a-b, Quantitative projection of pPIN1:PIN1-GFP signal at the meristem L1 (a) and patterns of pTAA1:TAA-GFP, pYUC1:GFP, and pYUC4:GFP expression (b) at the shoot apex show PIN1 upregulation coincident with predicted sites of auxin biosynthesis (arrowheads). Top views of meristems (a, b, upper panels) or leaf primordium (a, lower panel), and optical transverse sections through select leaf primordia (b, lower panels). Arrowheads, flanks of primordium-meristem boundaries where local PIN1 upregulation coincides with sites of auxin biosynthesis deduced from co-expression of TAA1, YUC1, and YUC4. Cell walls were stained by PI. Asterisks, meristem centre; p, leaf primordium. Scale bars, 20 µm. Representative images from n = 6 apices (a), and n = 10 apices per line (b) are shown.
