Extended Data Fig. 9: Incipient leaf primordia up to p-2 show a near uniform level of MP expression.
From: Specification of leaf dorsiventrality via a prepatterned binary readout of a uniform auxin input
a–c, Representative pattern of pMP:GFP-MP expression at the shoot apex showing a mostly uniform accumulation of MP at p-4 and p-3 incipient primordia. (a, b) Top view of the meristem (a) and leaf primordia (b). (c) Optical longitudinal sections at p-4 and p-3 leaf primordia. White, incipient primordium; asterisks, meristem centre. Cell walls were stained by PI. Scale bars, 50 µm (a), 20 µm (b, c). d, GFP-MP signal intensities measured along the apex radius at indicated primordium stages illustrate the near uniform level of MP accumulation. The distance 0 µm corresponds with the meristem centre. Positions of incipient primordia discerned by lineage tracing are marked in grey. Representative data from n = 10 apices (a) and n = 5 primordia per developmental stage (b-d) is shown.
