Extended Data Fig. 1: The spatial sequence of leaf primordia at the shoot apex reflects primordium development in time.
From: Specification of leaf dorsiventrality via a prepatterned binary readout of a uniform auxin input
a-b, A representative sequence of leaf primordium stages (b) at the shoot apex (a). Leaf primordia are numbered relative to the first bulging primordium (p1), numbers <1 indicate incipient primordia. Arrow, the first bulging primordium. c-d, Time-lapse series of the shoot apex (c) showing a temporal sequence of leaf development. Development of p-2 and p-1 primordia over 3 days (d) mirrors consecutive leaf primordia at the meristem. The p-2 and p-1 incipient primordia after 24 h attain developmental stages corresponding to p1 and p2, respectively (b), indicating a plastochron under the growth conditions used is approximately 8 h. Primordium stage numbers are maintained from the first time point. (a, c) Top views of the meristems. (b, d) Optical longitudinal sections through leaf primordia. Cell walls were stained by PI. Scale bars, 50 µm. Representative images from n = 6 apices (a, c) and n = 6 primordia (b, d) are shown.
