Extended Data Fig. 2: Genetic characterization of the bc18 mutant.
From: Xylan-based nanocompartments orchestrate plant vessel wall patterning
a, Map-based cloning of bc18. The letters on the chromosome indicate molecular markers for mapping. Number inside the parentheses indicate recombinant individuals. Arrows represent the genes within the mapping region. b, The mature plants of wild type (WT) and irx10-1. Bar = 12 cm. c, Western blotting of total membrane proteins extracted from the indicated plants using the anti-IRX10 antibodies. The reduced IRX10 signal in bc18 (a missense mutant) and absence of IRX10 signal in irx10-1 (a null mutant) demonstrated specificity of anti-IRX10 antibodies. Anti-BiP antibody was used for load control. d, Diagram of the IRX10 construct for complementation assay. e, Measurement of breaking force of the internodes in wild type, bc18, irx10-1, and the complementary line (Com). Error bars represent the mean ± s.d. n = 15 internodes from individual plants. P < 0.0001 by one-way ANOVA. Letters a–c indicate statistical significance according to Tukey’s multiple range test. P < 0.05. The experiment in (c) was repeated independently for three times.
