Extended Data Fig. 6: Transgenic complementation tests of tomato slclv3 single and slclv3 slcle9 double mutants.

a, b, Complementation tests of tomato slclv3 single mutants. Inflorescence images (a) and petal number quantifications (b) of WT and slclv3 compared to the T₁ transgenic plants gSlCLV3^SlCLV3, gPgCLE9^PgCLE9, gSlCLE9^PgCLE9, gSlCLV3^PgCLE9, and gSlCLV3^SlCLE9. c, Diagrams of the constructs used for complementation tests of slclv3 slcle9 double mutants. gSlCLV3^SlCLV3 (SlCLV3 genomic DNA). gSlCLV3^SlCLE9 (SlCLV3 genomic DNA including the sequence for SlCLE9 dodecapeptide). gSlCLE9^SlCLE9 (SlCLE9 genomic DNA). gSlCLE9^SlCLE9S6G (SlCLE9 genomic DNA including the sequence for PgCLE9 dodecapeptide). Black and orange rectangles mark the coding sequences and the dodecapeptide sequences, respectively. The numbers with minus (-) and plus (+) signs indicate the positions of the upstream sequences and the downstream sequences from the adenines of start codons, respectively. d, Carpel number quantifications of WT, slclv3, slclv3 slcle9 mutants compared to the T₁ transgenic plants gSlCLV3^SlCLV3-2, gSlCLV3^SlCLE9-2, gSlCLE9^SlCLE9-2, and gSlCLE9 ^SlCLE9S6G. Data are based on at least three independent transgenic lines for each construct. Box plots, 25^th-75^th percentile; centre line, median; whiskers, full data range in b and d. The letters indicate the significance groups at P < 0.01 (One-way ANOVA and Tukey test) in b and d. Different letters between genotypes indicate significance in b and d (See Supplementary Data 7 for specific P values). Exact sample sizes (n) are shown in b and d. At least twice experiments were repeated independently with similar results.