Extended Data Fig. 5: Groundcherry pgclv1 pgclv3 and petunia phclv1 phclv3 double mutants are severely fasciated like tomato slclv1 slclv3 double mutants.

a, Gene structure and sequences of two phclv1 CRISPR mutants. Guide RNA and PAM sequences are highlighted in red and bold underlined, respectively. Blue letters and dashes indicate insertions and deletions, respectively. Numbers in parentheses represent gap lengths. b, Flowers, fruits/pods, and meristems from pgclv1, phclv1, and slclv1 single mutants. White arrowheads mark petals or locules. 7L, 7^th leaf primordium, 8L, 8^th leaf primordium. 22L, 22^th leaf primordium. C, Side and top-down views of a pgclv1 pgclv3 double mutant shoot, inflorescence/floral meristem, and primary inflorescence. 6L, 6^th leaf primordium. D, Side and top-down views of a phclv1 phclv3 double mutant shoot and primary flower. E, Side and top-down views of a slclv1 slclv3 double mutant shoot, flower, vegetative meristem and primary inflorescence. Fasciated flowers and vegetative meristems are shown in insets of c and e. f, g, Petal (f) and locule (g) numbers of groundcherry WT, pgclv1, pgclv1 pgclv3, pgclv1 pgcle9, and petunia WT, phclv1, and tomato WT, slclv1, slclv1 slclv3, and slclv1 slcle9. Not that all three Solanaceae clv1 single mutant fasciation phenotypes are similarly weak. Box plots, 25^th-75^th percentile; centre line, median; whiskers, full data range in d and e. The letters indicate the significance groups at P < 0.01 (One-way ANOVA and Tukey test) in f and g. Different letters between genotypes indicate significance in f and g (See Supplementary Data 7 for specific P values). P values (two-tailed, two-sample t-test) in f and g. Exact sample sizes (n) are shown in f and g. At least twice experiments were repeated independently with similar results.