Fig. 3: Gene expression and phytohormone synthesis at different developmental stages of the seed of Cycas and the evolution of seed storage proteins.
From: The Cycas genome and the early evolution of seed plants
a, Heatmap showing relative expression of genes in 11 co-expression modules by WGCNA across 4 developmental stages of the seed: S1, unpollinated ovule; S2, early stage of pollinated ovule; S3, late stage of pollinated ovule; and S4, fertilized ovule. b, Quantification of eight plant phytohormone amounts in the same four developmental stages of the Cycas seed as above. The grey histogram represents the amount of hormone (n = 2 biologically independent experiments) and the error bar represents the standard error. c, Phylogeny of SSPs in some representative species in land plants. The SSPs analysed include germin-like protein (GLP), legumin-like SSP (l-SSP), vicilin-like SSP (v-SSP) and v-AMP. A maximum likelihood tree with 500 bootstrap replicates was constructed using RAxML. Bootstrap values (≥50%) for each major clade (highlighted in colour) and the relationships among them are provided. The Cycas sequences are highlighted in red. d, Expression levels of SSP in different tissues of C. panzhihuaensis.
