Fig. 2: Optimization of Arabidopsis leaf harvesting for measurement of ADPG.
From: Sucrose synthases are not involved in starch synthesis in Arabidopsis leaves
a–c, Rosettes were harvested from vegetative wild-type Col-0 plants (a) and from starch-deficient pgm (b) and adg1 (c) mutants in the light (white bars) by pouring liquid nitrogen (liq. N) directly onto the rosette or by cutting the hypocotyl from below and transferring the cut rosette into liquid nitrogen, taking care to avoid all shading. In parallel, other plants were deliberately shaded (grey bars) for 2–3 s before cutting and snap-freezing the rosettes. ADPG was measured by LC–MS/MS. Bars show mean ± s.d. of 5 independently pooled batches of 5 rosettes (n = 5 biological replicates). P values are shown for pairwise comparisons from a one-way analysis of variance (ANOVA) (Holm-Sidak post-hoc test). There were no significant differences between the samples in b (P = 0.285).
