Extended Data Fig. 4: Homology, filler, and T-DNA loss profiles for cNHEJ mutants.
From: Distinct mechanisms for genomic attachment of the 5′ and 3′ ends of Agrobacterium T-DNA in plants
Frequency of different lengths of microhomology (a - d), filler (e - h), or T-DNA loss (i - l) at RB and LB junctions, comparing wt (yellow) with cNHEJ mutants ku70 and lig4 (blue). The third colour in each panel indicates the overlapping area. The medians (dashed lines), the number of observations (n), and shifts in the mutant distribution relative to wt (s) are indicated. Wilcoxon rank-sum tests were performed to find the direction and the significance of the shifts (phomology_ku70_RB = 7 × 10−38, phomology_ku70_LB = 4 × 10−1, phomology_lig4_RB = 2 × 10−14, phomology_lig4_LB = 5 × 10−1, pfiller_ku70_RB = 4 × 10−1, pfiller_ku70_LB = 4 × 10−1, pfiller_lig4_RB = 4 × 10−3, pfiller_lig4_LB = 4 × 10−1, pdeletion_ku70_RB = 3 × 10−28, pdeletion_ku70_LB = 5 × 10−8, pdeletion_lig4_RB = 4 × 10−21, pdeletion_lig4_LB = 1 × 10−12). ns: p ≥ 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001.
