Extended Data Fig. 4: 4 k-mer based selection of target transcript regions with reduced off-target homology.
From: Foliar application of clay-delivered RNA interference for whitefly control
By minimizing the size of the union of 14nt canonical k-mer sets derived from an incrementing transcript window of 400nt and the off-target transcriptomes of Homo sapiens (human), Apis mellifera (European honeybee), Apis florea (dwarf honeybee), Apis dorsata (giant honeybee), Apis cerana (eastern honeybee) and Danaus plexippus (monarch butterfly), regions of least homology were identified. Within these regions, primers with 5’ T7 promoter sequences were designed via Primer3 to generate amplicons of approximately 300 bp. This approach allowed for rapid dsRNA design on a complete whitefly transcriptome basis.
