Extended Data Fig. 10: Uptake of abaxially-applied dsRNA by adult whiteflies on the abaxial surface of intact mature cotton leaves.
From: Foliar application of clay-delivered RNA interference for whitefly control
Cy3-labelled CMV2b dsRNA was applied with and without Banjo adjuvant on the abaxial (lower) surface of the cotton leaves and incubated at room temperature for 24 h. Adult whiteflies were released into the clip cage, also on the abaxial surface, and allowed to feed for a further 48 h. Confocal microscopy of the whole insect fed on dsRNA with or without adjuvant indicates localization of Cy3-labelled dsRNA in the whitefly gut, whereas control whiteflies fed on control (water) plants did not show a fluorescent signal. Bright-field (BF) image (column 1), Cy3 fluorescence image (column 2) and merged image of the two (column 3) are shown. Scale bar = 100 µm.
