Extended Data Fig. 5: BAM and CIK are required for CLE25/26/45-mediated regulation of phloem development.
a, Effects of CLE25, CLE26 and CLE45 peptides on the root growth of bam mutants. Plants were grown for 5 days on plates containing 10 nM peptides. Data are means ± s.e.m. Two-way ANOVA; peptide: F(3, 284) = 357.4, P < 0.0001; genotype: F(3, 284) = 441.1, P < 0.0001; interaction: F(9, 284) = 66.18, P < 0.0001; different letters indicate significant difference at P < 0.05 by Tukey’s test. n, biologically independent sample number. b, Effects of CLE25 on the root growth of cik2, cik3, and the cik2/3 double mutant. Data are means ± s.e.m. Two-way ANOVA; peptide: F(1, 89) = 87.98, P < 0.0001; genotype: F(3, 89) = 42.31, P < 0.0001; interaction: F(3, 89) = 18.94, P < 0.0001; different letters indicate significant difference at P < 0.05 by Sidak’s test. n, biologically independent sample number. c, Effects of CLE25 or CLE45 (synthetic peptide, 100 nM) on phloem development. Five-day-old plants on GM plates were moved onto plates containing CLE peptides for 2 days treatment. d, Effects of CLE25 or CLE45 overexpression on phloem development. Five-day-old two individual T2 lines of ER8::CLE25 and ER8::CLE45 were treated by 10 μM oestriol for 2 days. c,d, All plants contain APLpro::nls3GFP. Percent of plants that have phloem on two sides (green) and on one side (light green), and that have no phloem (open box). The presence of phloem was determined by the presence of the APLpro::nls3GFP signal and the SR2200 staining at newly grown parts after the start of treatment. Different letters indicate significant difference at P < 0.05 by two-sided Dunn’s test. n, biologically independent sample number.
