Extended Data Fig. 1: Phloem-Dofs positively regulate vascular cell file numbers.
a, The spatial pattern of gene expression mediated by ER8, which was used for overexpression in this study. The GFP signal of ER8::H2B-GFP was present in all cell types in the root tip, although expression in cortex was weak after 12 hours treatment of 10 µM oestriol. b,c, Non-xylem cell file number (b) and xylem cell file number (c) in wild-type vascular tissue (pericycle is not included), ER8::Dof1.1, and ER8::Dof1.1-SRDX grown in the presence of 100 nM oestriol. d, Representative images of (b) and (c). e,f, Non-xylem cell file number in 5-day-old wild-type and dof mutant plants, and a dof-sextuple mutant line transformed with PEAR1pro::PEAR1-mNG::PEAR1ter. g,h, Xylem cell file number in 5-day-old plants of the wild-type and dof mutants, and a dof-sextuple mutant line transformed with PEAR1pro::PEAR1-mNG::PEAR1ter. i, The expression pattern of PEAR1pro::PEAR1-mNG::PEAR1ter in the phloem region of 5-day-old dof-sextuple root vasculature. b,c,e–h, Data are means ± s.e.m. Different letters indicate significant difference at adjusted P < 0.05 by two-sided Tukey’s multiple comparisons test. n, numbers of biologically independent samples. Bars, 20 µm.
