Extended Data Fig. 3: TOP1α controls root tip homeostasis in response to sucrose.
From: TOP1α fine-tunes TOR-PLT2 to maintain root tip homeostasis in response to sugars
a-c, Quantification of QC cell number (a), CSC layer (b), and CC number (c) of WT and top1α ± Suc treatment. Data represent mean ± s.d. of 3 biological repeats (a, b). Data were analyzed from 15 seedlings for each treatment from 3 experiments (c). Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test (P < 0.05). d, RT-qPCR of CLE40 and CDF4 expression in WT and top1α under the indicated treatments. Gene expression in WT –Suc was set to 1. Data represent mean ± s.d. of 3 biological repeats. Student’s t test (two-tailed). e, Glucose contents in Ler and top1a. Statistical significance was determined by one-way ANOVA and Tukey test (P < 0.05). Data represent mean ±s.d. of 3 biological repeats (n = 3). Each replicate (n) is from 500–550 root tips. f. TOP1α expression patterns in pTOP1a::GFP root tips. Scale bar: 50 µm. g. TOP1α expression in pTOP1a::GFP root under indicated treatments. (m/n) indicates m in n of biological repeats showing the displayed features. Scale bar: 100 μm.
