Extended Data Fig. 4: Nuclear-localized PBL19 induces transcriptional self-amplification and defence-related gene expression.
a, RNA-seq analysis reveals that defence-related genes are upregulated in pbl19/pPBL19:PBL19C3A-GFP-HA plants. b, RNA-seq analysis reveals that PBL19 (red) is the most highly upregulated gene in the RLCK VII subfamily in PBL19C3A complementation plants. c, RT–qPCR assay confirms the upregulation of defence marker genes in PBL19C3A complementation plants. ACTIN2 was used as the reference gene for normalizing indicated gene expression. Data are shown as mean ± s.d. of three biological replicates with P values indicated (two-sided Student’s t-test). d, RNA-seq and ChIP–seq co-analysis reveals that PBL19 (red) is upregulated by PBL19C3A and meanwhile is bound by the nuclear-localized PBL19. e, PBL19 transcription is upregulated by chitin treatment for 30 min. ACTIN1 was used as the reference gene for normalizing PBL19 expression. Data are shown as mean ± s.d. of three biological replicates with P values indicated (two-sided Student’s t-test).
