Extended Data Fig. 7: WRKY8 and WRKY28 positively regulate plant antifungal immunity. | Nature Plants

Extended Data Fig. 7: WRKY8 and WRKY28 positively regulate plant antifungal immunity.

From: Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity

Extended Data Fig. 7

a, CRISPR-mediated knockout of WRKY8 and WRKY28 generates multiple null alleles. The protein length of wild-type or mutated WRKY8 and WRKY28 is indicated in the parenthesis. b,c, wrky8-c3 wrky28-c2 double mutant plants are more susceptible to V. dahliae infection. Disease severity in each datapoint was quantified by qPCR-based quantification of fungal biomass in pooled above-ground tissues of 32 plants from indicated genotype. Data are presented as boxplots with each dot representing the datapoint of one biological replicate. For the boxplots, the central line indicates the median, the bounds of the box show the 25th and the 75th percentiles, and the whiskers indicate 1.5 × interquartile range between the 25th and the 75th percentiles. P value was indicated (two-sided Student’s t-test). d, BiFC assay reveals that WRKY28 interacts with WRKY8 in protoplasts. ARF5 was used to replace WRKY8 as a negative control. Scale bar = 10 μm.

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