Extended Data Fig. 8: ntPBL19 and PBL19Δ1-24 interact with EDS1 in cytoplasmic punctate structures. | Nature Plants

Extended Data Fig. 8: ntPBL19 and PBL19Δ1-24 interact with EDS1 in cytoplasmic punctate structures.

From: Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity

Extended Data Fig. 8

a, BiFC assay reveals that EDS1 interacts with PBL19C3A in cytoplasmic punctate structures in protoplasts. The nucleocytoplasmic protein InLYP1 was used to replace PBL19C3A as a negative control. b, Coomassie brilliant blue (CBB) staining of IP-enriched ntPBL19 proteins from pbl19/pPBL19:PBL19C3A-GFP-HA plants. c, PBL19Δ1-24 is mainly localized to the cytoplasm in protoplasts. Scale bar = 10 μm. d, BiFC assay reveals that EDS1 interacts with PBL19Δ1-24 in cytoplasmic punctate structures in protoplasts. Scale bar = 10 μm. e, PBL19Δ1-24 proteins in multiple transgenic lines exhibit comparable molecular sizes to ntPBL19. Rubisco staining indicates comparable protein loading. f, PBL19Δ1-24 complementation plants are only slightly smaller than wild-type plants. Representative four-week-old plants are shown. Scale bar = 1 cm. g, CRISPR-mediated knockout of MC4~MC7 in PBL19C3A complementation plants. The experiments in (a), (c) and (d) were repeated three times and those in (b) and (e) were repeated twice with similar results.

Back to article page