Extended Data Fig. 9: Cytoplasmic accumulation of EDS1 is induced by treatment of chitin but not flg22.
a, The anti-EDS1 antibody can recognize endogenous EDS1 as a protein slightly bigger than 60 kDa. Rubisco staining indicates comparable protein loading. b. The anti-EDS1 antibody can recognize endogenous EDS1 that is co-immunoprecipitated with ntPBL19. c-e, Chitin induces cytoplasmic accumulation of endogenous EDS1 in a PBL19-dependent manner. Chitin-induced cytoplasmic enrichment of EDS1 only occurred in wild-type (c) and pbl19/pPBL19:PBL19-GFP-HA (e) plants but not in pbl19 null (d) plants. f, flg22 is unable to induce cytoplasmic accumulation of endogenous EDS1. In (c)–(f), the asterisks mark endogenous EDS1 in the blots. The abundances of EDS1 were quantified by Image J based on the densitometric ratios against tubulin (Input fraction), histone H3 (Nucleic-enriched fraction), and tubulin (Nucleic-depleted fraction), respectively. All experiments were repeated at least twice with similar results.
