Extended Data Fig. 1: PBL19 possesses a functional nuclear localization sequence. | Nature Plants

Extended Data Fig. 1: PBL19 possesses a functional nuclear localization sequence.

From: Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity

Extended Data Fig. 1

a, PBL19 contains a predicted palmitoylation site at Cys3 (blue) and a putative nuclear localization sequence (NLS) (red), whereas its paralogs PBL20 and BIK1 only have a palmitoylation site (blue). b, PBL20 and PBL20C3A are localized to the PM and cytoplasm, respectively, in protoplasts. HY5-Cherry is the nuclear marker. Scale bar = 10 μm. c, The NLS of PBL19 is functional. Note that the N-terminal fragment of PBL19 containing the NLS is sufficient to drive some cytosolic mCherry-GUS proteins into the nucleus. Dof1a-GFP is the nuclear marker. Scale bar = 10 μm. The experiments in (b) and (c) were repeated twice with similar results.

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