Extended Data Fig. 2: Chitin induces nuclear translocation of PBL19.
a, Chitin induces a fraction of PBL19 proteins to translocate from the PM to the nucleus. The same protoplasts transiently expressing PBL19-GFP were immobilized by the thin alginate layer and photographed before and after 1 h chitin exposure. The experiments were repeated twice with similar results. b,c, Biochemical fractionation indicates that nuclear translocation of PBL19 in transgenic plants only occurs under the elicitation of chitin (b) but not flg22 (c). d, Biochemical fractionation indicates that chitin does not elicit nuclear translocation of transiently expressed PBL20 in protoplasts.
